Edman degradation is performed on Procise 492 cLC (Applied Biosystems)

Analysis	Minimum Recommended Amount	Optimal Amount	Notes
N-terminal sequence analysis of proteins and peptides	2 pmol	10 – 50 pmol	Samples in solution: Solvent free of primary amines such as Tris or Gly Samples on PVDF membrane: Maximum membrane size is 15 – 20 mm2, whenever possible avoid Tris/Gly buffer for electroblotting