Proteomics > Which service should I request? > Characterization of purified biomolecules

Characterization of Purified Biomolecules

General description

After production and/or purification, proteins often need to be characterized for their integrity, the presence of natural or chemical modifications, or the assessment of sample heterogeneity. Antibody-drug conjugates, protein-metal complexes (i.e. metallothionein) and other biomolecules such as oligonucleotides can also be analyzed.
The determination of the mass of a protein/peptide is one way to confirm the identity of a protein and to provide insights into the presence of chemical / biological modifications. We offer analyses using (liquid chromatography) electrospray ionization (ESI) and matrix-assisted laser desorption/ionization (MALDI)-MS using routine or custom workflows.

List of applications and services

FGCZ offers the following types of MS-based analyses for the Characterization of Purified Biomolecules

Applications (examples) Which service to order
Determination of the molecular weight of intact proteins Protein / peptide mass determination
Confirmation of protein / peptide sequence Confirmation of protein identity
Characterization of protein disulfide bridgesDisulfide bridge assignment
Analysis of post-translational modifications (i.e. phospho, ubiquitin, acetyl, methyl) Analysis of selected PTMs
Characterization of protein-metal complexes (i.e. metallothioneins) Protein-metal complexes
Analysis of intact antibodies and antibody-drug conjugatesAntibody mass determination
Identification of purified protein N-Terminal N-terminal characterization (please contact us before ordering)
Characterization of oligonucleotide sequences (<25000 Da) Oligonucleotides mass determination
Structural analysis of small moleculesOther (only in special cases - please contact us before ordering)
Any analysis not covered in the list above Other (please contact us before ordering)

If you don't find what you are looking for, or you are interested in special applications, please contact us at proteomics at fgcz.ethz.ch

Created by paolo. Last Modification: 2023-03-31 15:47 by chiawei.