Proteomics > Which service should I request? > Characterization of purified biomolecules
The determination of the mass of a protein/peptide is one way to confirm the identity of a protein and to provide insights into the presence of chemical / biological modifications. We offer analyses using (liquid chromatography) electrospray ionization (ESI) and matrix-assisted laser desorption/ionization (MALDI)-MS using routine or custom workflows.
If you don't find what you are looking for, or you are interested in special applications, please contact us at proteomics at fgcz.ethz.ch
Characterization of Purified Biomolecules
Table of contents
General description
After production and/or purification, proteins often need to be characterized for their integrity, the presence of natural or chemical modifications, or the assessment of sample heterogeneity. Antibody-drug conjugates, protein-metal complexes (i.e. metallothionein) and other biomolecules such as oligonucleotides can also be analyzed.The determination of the mass of a protein/peptide is one way to confirm the identity of a protein and to provide insights into the presence of chemical / biological modifications. We offer analyses using (liquid chromatography) electrospray ionization (ESI) and matrix-assisted laser desorption/ionization (MALDI)-MS using routine or custom workflows.
List of applications and services
FGCZ offers the following types of MS-based analyses for the Characterization of Purified Biomolecules| Applications (examples) | Which service to order |
| Determination of the molecular weight of intact proteins | Protein / peptide mass determination |
| Confirmation of protein / peptide sequence | Confirmation of protein identity |
| Characterization of protein disulfide bridges | Disulfide bridge assignment |
| Analysis of post-translational modifications (i.e. phospho, ubiquitin, acetyl, methyl) | Analysis of selected PTMs |
| Characterization of protein-metal complexes (i.e. metallothioneins) | Protein-metal complexes |
| Analysis of intact antibodies and antibody-drug conjugates | Antibody mass determination |
| Identification of purified protein N-Terminal | N-terminal characterization (please contact us before ordering) |
| Characterization of oligonucleotide sequences (<25000 Da) | Oligonucleotides mass determination |
| Structural analysis of small molecules | Other (only in special cases - please contact us before ordering) |
| Any analysis not covered in the list above | Other (please contact us before ordering) |
If you don't find what you are looking for, or you are interested in special applications, please contact us at proteomics at fgcz.ethz.ch
Quick links
- How to order an analysis?
- Follow our order instructions
- Questions?
- Check our FAQs page or contact us at proteomics at fgcz.ethz.ch
- Prices
- The prices for the most common proteomics services can be found here.