Info Sheet: ChIP-seq

How to submit samples for Illumina/Short Read Sequencing.pdf

Workflow

1. QC: DNA quantification using Qubit
2. Library construction using the NEBNext Ultra DNA Library Prep Kit
3. Library QC
4. Sequencing
5. Bioinformatics data analysis (optional)

expected turnaround time: 6 weeks after passing QC

Input requirements

• at least 10 ng DNA
• at least 10 ul sample volume
• concentration must be 10 ng / ul - 100 ng /ul

1.5 ml tubes labeled with B-Fabric Tube ID, normalized to the same concentration

Experimental design

matching input control

Sequencing options

Read types (other configurations on request):

NovaSeq 6000

100 bp single-end reads

Recommended sequencing depth:

10 Mio reads / sample for transcription factors
50 Mio reads / sample for chromatin modifications

Costs for UZH und ETH research groups

Library prep	128 CHF / sample
200M reads	760 CHF
Bioinformatics package	880 CHF

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