Info Sheet: ChIP-seq
How to submit samples for Illumina/Short Read Sequencing.pdf
Workflow
- QC: DNA quantification using Qubit
- Library construction using the NEBNext Ultra DNA Library Prep Kit
- Library QC
- Sequencing
- Bioinformatics data analysis (optional)
Input requirements
- at least 10 ng DNA
- at least 10 ul sample volume
- concentration must be 10 ng / ul - 100 ng /ul
Experimental design
matching input controlSequencing options
Read types (other configurations on request):NovaSeq 6000 | 100 bp single-end reads |
Recommended sequencing depth:
10 Mio reads / sample for transcription factors50 Mio reads / sample for chromatin modifications
Costs for UZH und ETH research groups
Library prep | 128 CHF / sample |
200M reads | 760 CHF |
Bioinformatics package | 880 CHF |
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