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FAQs > FAQ - Glycan / Glycopeptide analysis
FAQs - Glycan / Glycopeptide analysis
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Glycan / Glycopeptide analysis page?
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[+] Do you offer both N- and O-glycopeptide analysis?
Yes, for N-glycopeptide sequencing, the workflow is mature and standardized and the N-glycosylation sequon is well known. Therefore, the validation of glycosylation sites is straightforward. However, O-linked glycans are more labile than N-linked ones so that the validation of glycosylation site is more difficult than N-linked glycans and requires more time and presence of key fragment ions.
[+] How much material is required for glycopeptide analysis/ site-specific glycosylation?
To obtain the whole profile of glycosylation on proteins, the purity of glycoproteins is crucial because glycopeptides are larger and more difficult to ionize than normal peptides. If the glycoprotein is the major component in the mixture, 10-50 µg of protein is sufficient for the analysis. If not, please contact us to get more instruction.
[+] What will FGCZ provide as results of glycopeptide analysis/ site-specific glycosylation?
We will provide Byonic results and a summary of each glycosylation site.
[+] Do you offer large scale glycopeptide profiling?
Yes or No. The glycoproteomic scale study requires enrichment steps, data analysis power and better experimental planning. If you have any requests, please contact us.
[+] How much material is required for glycomic analysis?
It depends on your materials.
If it is a pure protein sample -> 10-100 µg is sufficient.
If it is cell lines -> minimal 5 million cells.
If it is other glycan samples -> please contact us
[+] What will FGCZ provide as results of glycan analysis?
The peak list files and annotated structures will be provided in the report.
[+] Do you offer monosaccharide analysis?
Yes, we do offer monosaccharide analysis, but only for mammalian glycans. All details
here