FLX Sample Requirements

Sample Requirements

Type of library	Optimal amount (µg)	Concentration (ng/µl)
Fragment/Shotgun	0.5	>50
3 kb span Paired-End	5	>50
8 kb span Paired-End	15	>100
20 kb span Paired-End	30	>100
Amplicon	>0.1	>10

• DNA quantitation must be done by fluorometry (e.g. PicoGreen-based). If the concentration of a sample is lower than specified please speed vac before submission.
• Purified samples with an A260/A280 of ~1.8 can be provided in water or Qiagen EB buffer.
• Samples for Paired-End sequencing should not contain EDTA. Elute them in 10 mM Tris-HCl, pH 7.5-8.5.
• Please provide an image of a gel for each sample with clearly indicated sample identity and ladder bands size or Bioanalyzer electropherogram.

IMPORTANT: For data QC expected primer sequences must be e-mailed to FGCZ. For mutations detection against a reference the reference sequences must also be sent to FGCZ.

Submitted samples will be stored for four weeks after data delivery. During this time the material unused for analysis can be taken back. After this period, any biological material will be discarded without further notification.