Sequencing > Services
Exome sequencing
Targeted resequencing
ChIP seq
Amplicon Sequencing
miRNA profiling
illumina MiSeq Personal Sequencer
FGCZ MiSeq Sequencing
Contact sequencing at fgcz.ethz.ch for planning your experiment.Applications
Small Genome SequencingExome sequencing
Targeted resequencing
ChIP seq
Amplicon Sequencing
miRNA profiling
Sequencing Throughput Specifications:
Read Length (bp) | Number and Type of Reads | Throughput
|
1 x 36 (for smRNA seq) | 15-17 M Single reads | 540-610 Mb |
2 x 25 | 20-25 M Paired-End reads | 750-850 Mb |
2 x 100 | 20-25 M Paired-End reads | 3.0-3.4 Gb |
2 x 150 | 20-25 M Paired-End reads | 4.5-5.1 Gb |
2 x 250 | 20-25 M Paired-End reads | 7.5-8.5 Gb |
2 x 300 | 20-25 M Paired-End reads | 13-15 Gb |
Library Preparation
One can use the following standard protocols:- Illumina Library kits
- Nextera Kits
- Nextera XT for dual indexing
When do you opt for MiSeq
- To acheive speed (Maximum run time is 40hrs)
- When longer read lengths are required (2 x 300bp)
- When the throughput required is lower than 10Gb
- Want to sequence several plasmids in one go with 96 Barcodes (Using Nextera XT dual indexing)