Proteomics > Proteomics User Lab
The course includes both self-paced and on-site learning modules, covering a theoretical and a practical introduction to mass spectrometry-based proteomics. At the end of the course, each user will receive an "Operator License" certificate, who will give access to our LC-MS instruments, analytical equipment, and software infrastructure.
Alternatively, please contact the coach of your FGCZ project!
FGCZ Proteomics User Lab
Table of contents
User Lab Guidelines
FGCZ User GuidelinesConcept
The Proteomics User Lab is offered to UZH/ETH users, who- plan to perform long term proteomics experiment (at least over 1 year, running multiple experiments)
- design mass spectrometry-intense projects (e.g., with 100s of samples)
- are interested in non-standard protocols that we do not offer in service mode
- are motivated to develop new protocols
User Lab Course - Operator license
As of January 2022, we improved the structure of our trainings and all new users are requested to complete a short User Lab Course prior to starting a new project.The course includes both self-paced and on-site learning modules, covering a theoretical and a practical introduction to mass spectrometry-based proteomics. At the end of the course, each user will receive an "Operator License" certificate, who will give access to our LC-MS instruments, analytical equipment, and software infrastructure.
How to enroll
If you do not have any running FGZC project, contact us at proteomics at fgcz.ethz.ch. You will be asked to submit a project in our B-Fabric data management systemAlternatively, please contact the coach of your FGCZ project!
Modules
The course consists of the following modules:| Module number | Title | Topics | Type | Estimated duration | Notes |
| M1 | Project management using B-Fabric | Basic concepts of an user lab project at FGCZ; Functionalities of our data management system | self-paced learning + self-assessment | ~2h | |
| M2 | Introduction to proteomics and the basics of sample preparation | Typical proteomics workflows; Basics of mass spectrometry | self-paced learning + self-assessment, accessible only once M1 is completed) | ~2h | |
| M3 | LC-MS Operations | Start an LC-MS system and check the performances; Access other instruments (i.e., HPLC, KingFisher) | in person, instructor led -accessible only once M1/M2 are completed | 1 day | 2-3 persons |
| M4 | Data analysis | Accessing the software infrastructure at FGCZ; Basics of protein identification and quantification | self-paced learning + self-assessment, accessible only once M3 is completed) | 3-5h | |
| M5 | Data analysis Q&A | Dedicated time to discuss open data analysis questions | in person, instructor led -accessible only once M4 is completed | ~2-3h | |
| M6 | Final test | Test consisting of practical assignments and free text answers | self-paced | 1-3h (plus computational time) |
Dates
The course is offered as follow:- Once a month (independently of the number of requesters)
- or, as soon as 2 new users want to start a new project.