Proteomics > Proteomics User Lab
The course includes both self-paced and on-site learning modules, covering a theoretical and a practical introduction to mass spectrometry-based proteomics. At the end of the course, each user will receive an "Operator License" certificate, who will give access to our LC-MS instruments, analytical equipment, and software infrastructure.
Alternatively, please contact the coach of your FGCZ project!
FGCZ Proteomics User Lab
Table of contents
Concept
The Proteomics User Lab is offered to UZH/ETH users, who- plan to perform long term proteomics experiment (at least over 1 year, running multiple experiments)
- design mass spectrometry-intense projects (e.g., with 100s of samples)
- are interested in non-standard protocols that we do not offer in service mode
- are willing to develop new protocols
User Lab Course - Operator license
As of January 2022, we improved the structure of our trainings and all new users are requested to complete a short User Lab Course prior to starting a new project.The course includes both self-paced and on-site learning modules, covering a theoretical and a practical introduction to mass spectrometry-based proteomics. At the end of the course, each user will receive an "Operator License" certificate, who will give access to our LC-MS instruments, analytical equipment, and software infrastructure.
How to enroll
If you do not have any running FGZC project, contact us at proteomics at fgcz.ethz.ch. You will be asked to submit a project in our B-Fabric data management systemAlternatively, please contact the coach of your FGCZ project!
Modules
The course consists of the following modules:| Module number | Title | Topics | Type | Estimated duration | Notes |
| M1 | Project management using B-Fabric | Basic concepts of an user lab project at FGCZ; Functionalities of our data management system | self-paced learning + self-assessment | ~2h | |
| M2 | Introduction to proteomics and the basics of sample preparation | Typical proteomics workflows; Basics of mass spectrometry | self-paced learning + self-assessment, accessible only once M1 is completed) | ~2h | |
| M3 | LC-MS Operations | Start an LC-MS system and check the performances; Access other instruments (i.e., HPLC, KingFisher) | in person, instructor led -accessible only once M1/M2 are completed | 1 day | 2-3 persons |
| M4 | Data analysis | Accessing the software infrastructure at FGCZ; Basics of protein identification and quantification | self-paced learning + self-assessment, accessible only once M3 is completed) | 3-5h | |
| M5 | Data analysis Q&A | Dedicated time to discuss open data analysis questions | in person, instructor led -accessible only once M4 is completed | ~2-3h | |
| M6 | Final test | Test consisting of practical assignments and free text answers | self-paced | 1-3h (plus computational time) |
Dates
The course is offered as follow:- Once a month (independently of the number of requesters)
- or, as soon as 2 new users want to start a new project.