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SOLiD 4 System

Applied Biosystems website contains the latest information on SOLiD System technology.

Using FGCZ NGSequencing Services

SOLiD Samples Submission form and detailed sample requirements.

Before submitting a sample, be aware of the following SOLiD recommendations regarding samples preparation and quality.


Quantitate the samples by fluorometry using the Quant-iT DNA assay kit (Invitrogen), following the manufacturer's instructions.
Although various quantitation methods are possible, fluorometry has been found to be highly reproducible and to provide consistent emPCR and sequencing results.

New SOLiD 5000xl System exclaim



The SOLiD 4 technology supports
  • Fragment Sequencing (50 bp, 35 bp) also with Barcodes (currently 96 available)
  • Paired End 50/25 bp, 50/35 bp (96 barcodes available)
  • Mate-Paired 2 x 50 bp and 2 x 25 bp, 2 x 35 bp
  • Whole Transcriptome Analysis (48 barcodes available)
  • Small RNA Expression (48 barcodes available)
  • SAGE - genome-wide expression

Sequencing Throughput

The table below lists the approximate maximum sequencing throughput for each loading region, and the corresponding total throughput for sequencing slide for re-sequencing with Fragment Library. The throughput for other types of libraries is lower.

Loading Region
Size
# Regions
per Slide
Mappable reads
(M)
Throughput with
50 bp reads (Gb)
per region per slide
Image Full slide
High Density		 1 350 17.5 17.5
Image Quart
High Density		 4 64 3.2 12.8
Image Oct
High Density		 8 28 1.4 11.2

Applications

ChIP-Seq with SOLiD System

Amplicon sequencing

SOLiD Fragment Library Barcoding Kit 1–96 Manual contains sequence of sequencing adapter P1 and adapters P2 with sequencing barcode
see page 25
P1: Multiplex Library PCR-1, 50 μM 5′-CCACTACGCCTCCGCTTTCCTCTCTATGGGCAGTCGGTGAT-3′
P2: with Barcode-001, 50 μM 5′-CTGCCCCGGGTTCCTCATTCTCTGTGTAAGAGGCTGCTGTACGGCCAAGGCG-3′

SOLiD Fragment Library not barcoded
P1 adapter: 5'-CCACTACGCCTCCGCTTTCCTCTCTATGGGCAGTCGGTGAT-3'
P2 adapter: 5'-AGAGAATGAGGAACCCGGGGCAGTT 3'

Construct of the fragment library with P1 adapter, NNN... sequence of the read, and P2 adapter (without barcode):
5' CCACTACGCCTCCGCTTTCCTCTCTATGGGCAGTCGGTGAT-NNNNNNN--NNNNNNN AGAGAATGAGGAACCCGGGGCAGTT 3'