LPS Microarrays

In collaboration with Prof. Thiery Hennet (Institute of Physiology, UZH), we offer high quality microarrays with spotted 130 lipopolysaccharides, and service for slide processing, scanning, and data evaluation. LPS were extracted and purified from Gram-negative bacteria and cover species including Aeromonas, Bacteroides, Citrobacter, E.Coli, Hafnia, Klebsiella, Pseudomonas, Salmonella, Yersinia, and serotypes thereof.

Slides are produced with a state-of-the-art microarray spotter (Nanoplotter NP2.1, GeSiM, Dresden) and contain 9 replicate spots per LPS probe. Slide quality control is carried out by incubating a number of slides per batch with a standard (serum) sample followed by a secondary dye labeled antibody specific for IgG and IgM, resp., for fluorescence detection.

Procedure

If you want to conduct experiments with LPS arrays we invite you to come to the biophysics lab of the FGCZ in Zurich. Here we incubate slides with your samples in an automated slide processor (hybridisation station HS4800, Tecan), scan the processed slides using a confocal microscope (LS400, Tecan), and evaluate the scan data. Alternatively, you can send your samples for processing.
Upon request, we offer - as an special service - the spotting of your specific controls to the array of LPS.

If you are interested, please get in touch with Dr. Jens Sobek (jens.sobek@fgcz.ethz.ch) for more information and quote request.

Examples

The advantage of processing slides in an automated processor is the full control over protocol parameters, fewer processing artifacts, and a homogeneous background. Scan images were converted from 16 bit tiff to 8 bit jpg with no further processing.

Figure 1

Figure 1: Scans of an LPS microarrays v1.20. The slide was incubated with a serum sample, the fluorescence was detected with an IgA and IgG antibody.

Figure 2

Figure 2: Signal-to-noise ratio for IgA detection (detail of the IgA high voltage scan, Fig. 1). For high intensity signals, S/N ratio is > 1000.

Figure 3

Figure 4

Figs.3,4: Scans of an LPS microarray v1.30 incubated with a serum sample detected with an IgG and IgM antibody. The white spots are positive controls.