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FGCZ Genomics > Equipment




Short-read sequencers


SequencersIlumina NovaSeqXIlumina NovaSeq Ilumina NextSeq 2000 llumina NextSeq 500 Ilumina MiSeq Ilumina MiniSeqIlumina iSeq Element Aviti
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Flowcell 25B, 10B, 1.5B SP, S1,S2,S4 P2, P3 High Output, Mid output High Output, Mid output High Output, Mid output High Output, Mid output
Key Features Patterned Flowcell Patterned Flowcell Patterned Flowcell Non-Patterned Flowcell Non-Patterned Flowcell Non-Patterned Flowcell Nanowells, CMOS Nanoballs
Reads 10B Reads per FC S4 8 Billion P3 1.1B HO 350 Million 25 Million HO 22 Million 4Million HO 1B reads, MO 500M reads
SP 650-800M P2 400M MO120 Million MO 7 Million LO 250M reads
Read Length 2X 150bp, 1X100bp 2X150 bp, SP 2X250bp 2X150 bp, 2X 250bp 2X150 bp 2X300 bp 2X150 bp 2X150 bp 2X300 bp, 2X150bp
Approx Price per Gb S4-12.10 chf, S1-25.9 chf 46.5 chf 196.8 chf 282 chf 925 chf
Key Applications Large WGS, RNA Seq Large WGS, RNA Seq Single cell SequencingATAC Seq, ChIP Small WGS, Amplicons Small WGS, QCs Small WGS Large WGS, RNA Seq
Single Cell, Spatial Custom primers small RNA Targeted Sequencing, QCs small RNA, amplicons
Access Mode Service Service Walk-in Service, Walk-in Service, Walk-in Service, Walk-in Service, Walk-in Service, Walk-in
Location Irchel Campus Irchel Campus GEML Honggerberg Irchel Campus Irchel Campus Irchel Campus Irchel Campus Irchel Campus
Irchel Campus GEML Honggerberg

Long-read sequencers


Pacbio Sequel IIe ONT P2 Solo ONT GridION X5 Promethion 24 Pacbio Revio
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Inhouse Inhouse Inhouse Inhouse Outsourced
Cell Type8M SMRT Cell PromethION R9.4 Minion PromethION R9.4, 10.4.1 25M SMRT Cell
gDNA
av. pol read length 15+ Kb 10-30 Kb 10-30 Kb 10-30 Kb 15+ Kb
expected output 70- 90+ Gb 30-90 Gb up to 1 Gb 4-15 Gb 280 Gb
output HiFi mode 20+ Gb - - -
Amplicons/cDNA
av. read length* 20+ Kb 1-10Kb 1-10 Kb 1-10 Kb 17 Kb
number of reads 4-6 M 40-70 M 0.1-0.5 M 1-10 M 12 M
number of HiFi reads 1.5-2.5 M - - - 7.5M


Single cell instruments


Instruments 10X Chromium X 10X Chromium BD Rhapsody Takara Icell8
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Application Single Cell Single Cell Single Cell Single Cell RNA and DNA
Number of cells 10Kcells 10K cells 25K cells
Sample Type Fresh, Fixed cells Fresh Cells Fresh Cells


Spatial transcriptomics instruments


Instruments 10X Cytassist 10X Xenium
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Application Spatial Spatial
Number of cells 6X6, 11X11 12mm x 24 mm
Sample Type FF, FFPE, FixedFrozen FF, FFPE


Created by hubert. Last Modification: 2024-05-23 13:55 by hubert.