Sequencing > Equipment

Short-read sequencers

Sequencers	Ilumina NovaSeq	Ilumina NextSeq 2000	llumina NextSeq 500	Ilumina MiSeq	Ilumina MiniSeq	Ilumina iSeq
Flowcell	SP, S1,S2,S4	P2, P3	High Output, Mid output	High Output, Mid output	High Output, Mid output
Key Features	Patterned Flowcell	Patterned Flowcell	Non-Patterned Flowcell	Non-Patterned Flowcell	Non-Patterned Flowcell	Nanowells, CMOS
Reads (SE) per FC	S4 8 Billion	P3 1.1B	HO 350 Million	25 Million	HO 22 Million	4Million
	SP 650-800M	P2 400M	MO120 Million		MO 7 Million
Max Read Length	2X150 bp, SP 2X250	2X150 bp	2X150 bp	2X300 bp	2X150 bp	2X150 bp
Approx Price per Gb	S4-12.10 chf, S1-25.9 chf		46.5 chf	196.8 chf	282 chf	925 chf
Key Applications	Large WGS, RNA Seq	Single cell Sequencing	ATAC Seq, ChIP	Small WGS, Amplicons	Small WGS, QCs	Small WGS
	Single Cell		small RNA			Targeted Sequencing, QCs
Access Mode	Service	Walk-in	Service, Walk-in	Service, Walk-in	Service, Walk-in	Service, Walk-in
Location	Irchel Campus	GEML Honggerberg	Irchel Campus	Irchel Campus	Irchel Campus	Irchel Campus
		Irchel Campus		GEML Honggerberg

Long-read sequencers

Long Read Sequencing instruments

	Pacbio Sequel IIe	ONT P2 Solo	ONT GridION X5
	Inhouse	Inhouse	Inhouse
Cell Type	8M SMRT Cell	PromethION R9.4	Flongle	MinION R9.4
gDNA
av. pol read length	15+ Kb	10-30 Kb	10-30 Kb	10-30 Kb
expected output	70- 90+ Gb	30-90 Gb	up to 1 Gb	4-15 Gb
output HiFi mode	20+ Gb	-	-	-
Amplicons/cDNA
av. read length*	20+ Kb	1-10Kb	1-10 Kb	1-10 Kb
number of reads	4-6 M	40-70 M	0.1-0.5 M	1-10 M
number of HiFi reads	1.5-2.5 M	-	-	-