FGCZ Genomics > Services

NGS-based Workflow Full Service


We offer a Full Service option for the standard applications listed below using validated library prep methods. We have an average turnaround time of about 4 weeks after the samples pass QC. If your sample doesn't reach the sample requirements of our standard protocols, we can suggest other protocols and we can continue with the experiment via our custom service mode or user lab mode.
You can find how to access our User Lab HERE

Standard Service Offerings, Applications and Sample Requirements:


Applications Protocols Offered Optimum Input Requirements Standard Sequencing Recommendations
1. mRNA Sequencing Illumina TruSeq mRNA Stranded 600 ng - 1 μg Total RNA, DNAse treated, column purified 20M reads, single read 100 bp
2. Total RNA Sequencing Illumina TruSeq TotalRNA Stranded (HMR) 600 ng - 1 μg Total RNA, DNAse treated, column purified 40M reads, single read 100 bp
3. Low Input RNA Sequencing Clontech Picomammalian, SmartSeq2 less than 10 ng Total RNA 20-40M reads, single read 100 bp
4. Small RNA Sequencing RealSeq 1 ng - 1 μg Total RNA, DNAse treated 10M reads, single read 75 bp
5. Single-cell Multiomics Sequencing 10X Genomics, BD Rhapsody 50’000 viable cells in a tube50’000 reads per cell
6. Whole Genome Sequencing TruSeq DNA Nano, TruSeq PCR Free, NEB Ultra II 600 ng - 1'000 ng high MW genomic DNA 30X coverage, paired end 150 bp
7. Low Input Whole Genome Sequencing NEB Ultra II, Nextera XT 1 - 10 ng high MW genomic DNA 30X coverage, paired end 150 bp
8. Whole Small Genome Sequencing Nextera XT 1 - 10 ng genomic DNA paired end 150 bp
9. Metagenomics Amplicon Barcoding inquire inquire
10. ChIP Seq NEB Ultra 10 ng Single ChIP enriched DNA or input DNA 20M reads per sample, single read 100 bp
11. Ready-Made Libraries 10 nM, pooled custom
12. Spatial Gene Expression 10X Visium FFPE custom


Please click on the application or protocol of your choice for a more detailed information (ie sample requirements, experimental design considerations).
Illumina Sequencing Pricing
How to submit samples for Illumina/Short Read Sequencing.pdf
How to submit Ready-made Libraries.pdf

Short-read sequencers


SequencersIlumina NovaSeqXIlumina NovaSeq Ilumina NextSeq 2000 llumina NextSeq 500 Ilumina MiSeq Ilumina MiniSeqIlumina iSeq Element Aviti
Image Image Image Image Image Image Image Image
Flowcell 25B, 10B, 1.5B SP, S1,S2,S4 P2, P3 High Output, Mid output High Output, Mid output High Output, Mid output High Output, Mid output
Key Features Patterned Flowcell Patterned Flowcell Patterned Flowcell Non-Patterned Flowcell Non-Patterned Flowcell Non-Patterned Flowcell Nanowells, CMOS Nanoballs
Reads 10B Reads per FC S4 8 Billion P3 1.1B HO 350 Million 25 Million HO 22 Million 4Million HO 1B reads, MO 500M reads
SP 650-800M P2 400M MO120 Million MO 7 Million LO 250M reads
Read Length 2X 150bp, 1X100bp 2X150 bp, SP 2X250bp 2X150 bp, 2X 250bp 2X150 bp 2X300 bp 2X150 bp 2X150 bp 2X300 bp, 2X150bp
Approx Price per Gb S4-12.10 chf, S1-25.9 chf 46.5 chf 196.8 chf 282 chf 925 chf
Key Applications Large WGS, RNA Seq Large WGS, RNA Seq Single cell SequencingATAC Seq, ChIP Small WGS, Amplicons Small WGS, QCs Small WGS Large WGS, RNA Seq
Single Cell, Spatial Custom primers small RNA Targeted Sequencing, QCs small RNA, amplicons
Access Mode Service Service Walk-in Service, Walk-in Service, Walk-in Service, Walk-in Service, Walk-in Service, Walk-in
Location Irchel Campus Irchel Campus GEML Honggerberg Irchel Campus Irchel Campus Irchel Campus Irchel Campus Irchel Campus
Irchel Campus GEML Honggerberg


Questions? Please email sequencing@fgcz.ethz.ch.
phone: 063 44 6353911 (For pressing issues only please. We prefer if you email us because we are mostly working in the lab so we can't always attend to phone calls)