FGCZ Genomics > Services

NGS-based Workflow Full Service

We offer a Full Service option for the standard applications listed below using validated library prep methods. We have an average turnaround time of about 4 weeks after the samples pass QC. If your sample doesn't reach the sample requirements of our standard protocols, we can suggest other protocols and we can continue with the experiment via our custom service mode or user lab mode.
You can find how to access our User Lab HERE

Standard Service Offerings, Applications and Sample Requirements:

Applications	Protocols Offered	Optimum Input Requirements	Standard Sequencing Recommendations
1. mRNA Sequencing	Illumina TruSeq mRNA Stranded	600 ng - 1 μg Total RNA, DNAse treated, column purified	20M reads, single read 100 bp
2. Total RNA Sequencing	Illumina TruSeq TotalRNA Stranded (HMR)	600 ng - 1 μg Total RNA, DNAse treated, column purified	40M reads, single read 100 bp
3. Low Input RNA Sequencing	Clontech Picomammalian, SmartSeq2	less than 10 ng Total RNA	20-40M reads, single read 100 bp
4. Small RNA Sequencing	RealSeq	1 ng - 1 μg Total RNA, DNAse treated	10M reads, single read 75 bp
5. Single cell Multiomics Sequencing	10X Genomics, BD Rhapsody	50’000 viable cells in a tube	50’000 reads per cell
6. Whole Genome Sequencing	TruSeq DNA Nano, TruSeq PCR Free, NEB Ultra II	600 ng - 1'000 ng high MW genomic DNA	30X coverage, paired end 150 bp
7. Low Input Whole Genome Sequencing	NEB Ultra II, Nextera XT	1 - 10 ng high MW genomic DNA	30X coverage, paired end 150 bp
8. Whole Small Genome Sequencing	Nextera XT	1 - 10 ng genomic DNA	paired end 150 bp
9. Metagenomics	Amplicon Barcoding	inquire	inquire
10. Chip Seq	NEB Ultra	10 ng Single ChIP enriched DNA or input DNA	20M reads per sample, single read 100 bp
11. Ready-Made Libraries		10 nM, pooled	custom
12. Spatial Gene Expression	10X Visium	FFPE		custom

Please click on the application or protocol of your choice for a more detailed information (ie sample requirements, experimental design considerations).
Illumina Sequencing Pricing
How to submit samples for Illumina/Short Read Sequencing.pdf
How to submit Ready-made Libraries.pdf

Short-read sequencers

Sequencers	Ilumina NovaSeqX	Ilumina NovaSeq	Ilumina NextSeq 2000	llumina NextSeq 500	Ilumina MiSeq	Ilumina MiniSeq	Ilumina iSeq	Element Aviti
Flowcell	25B, 10B, 1.5B	SP, S1,S2,S4	P2, P3	High Output, Mid output	High Output, Mid output	High Output, Mid output		High Output, Mid output
Key Features	Patterned Flowcell	Patterned Flowcell	Patterned Flowcell	Non-Patterned Flowcell	Non-Patterned Flowcell	Non-Patterned Flowcell	Nanowells, CMOS	Nanoballs
Reads	10B Reads per FC	S4 8 Billion	P3 1.1B	HO 350 Million	25 Million	HO 22 Million	4Million	HO 1B reads, MO 500M reads
		SP 650-800M	P2 400M	MO120 Million		MO 7 Million	LO 250M reads
Read Length	2X 150bp, 1X100bp	2X150 bp, SP 2X250bp	2X150 bp, 2X 250bp	2X150 bp	2X300 bp	2X150 bp	2X150 bp	2X300 bp, 2X150bp
Approx Price per Gb		S4-12.10 chf, S1-25.9 chf		46.5 chf	196.8 chf	282 chf	925 chf
Key Applications	Large WGS, RNA Seq	Large WGS, RNA Seq	Single cell Sequencing	ATAC Seq, ChIP	Small WGS, Amplicons	Small WGS, QCs	Small WGS	Large WGS, RNA Seq
	Single Cell, Spatial	Custom primers		small RNA			Targeted Sequencing, QCs	small RNA, amplicons
Access Mode	Service	Service	Walk-in	Service, Walk-in	Service, Walk-in	Service, Walk-in	Service, Walk-in	Service, Walk-in
Location	Irchel Campus	Irchel Campus	GEML Honggerberg	Irchel Campus	Irchel Campus	Irchel Campus	Irchel Campus	Irchel Campus
			Irchel Campus		GEML Honggerberg

Questions? Please email sequencing@fgcz.ethz.ch.
phone: 063 44 6353911 (For pressing issues only please. We prefer if you email us because we are mostly working in the lab so we can't always attend to phone calls)