Sequencing > Services

Illumina Workflow Full Service

We offer a Full Service option for the standard applications listed below using validated library prep methods. We have an average turnaround time of about 4 weeks after the samples pass QC. If your sample doesn't reach the sample requirements of our standard protocols, we can suggest other protocols and we can continue with the experiment via our custom service mode or user lab mode.
You can find how to access our User Lab HERE

Standard Service Offerings, Applications and Sample Requirements:

Applications	Protocols Offered	Optimum Input Requirements	Standard Sequencing Recommendations
1. mRNA Sequencing	Illumina TruSeq mRNA Stranded	600 ng - 1 μg Total RNA, DNAse treated, column purified	20M reads, single read 100 bp
2. Total RNA Sequencing	Illumina TruSeq TotalRNA Stranded (HMR)	600 ng - 1 μg Total RNA, DNAse treated, column purified	40M reads, single read 100 bp
3. Low Input RNA Sequencing	Clontech Picomammalian, SmartSeq2	less than 10 ng Total RNA	20-40M reads, single read 100 bp
4. Small RNA Sequencing	RealSeq	1 ng - 1 μg Total RNA, DNAse treated	10M reads, single read 75 bp
5. Single cell RNA Sequencing Droplet Based	10X Genomics Single Cell	1’000 - 10’000 viable cells in a tube.	50’000 reads per cell
6. Single cell RNA Sequencing Plate Based	Smartseq2 384 well plate Single Cell	Cells sorted in 384 well plates	1M reads per cell
7. Whole Genome Sequencing	TruSeq DNA Nano, TruSeq PCR Free, NEB Ultra II	600 ng - 1'000 ng high MW genomic DNA	30X coverage, paired end 150 bp
8. Low Input Whole Genome Sequencing	NEB Ultra II, Nextera XT	1 - 10 ng high MW genomic DNA	30X coverage, paired end 150 bp
9. Whole Small Genome Sequencing	Nextera XT	1 - 10 ng genomic DNA	paired end 150 bp
10. Metagenomics	Amplicon Barcoding	inquire	inquire
11. Chip Seq	NEB Ultra	10 ng Single ChIP enriched DNA or input DNA	20M reads per sample, single read 100 bp
12. Ready-Made Libraries		10 nM, pooled	custom
13. Spatial Transcriptomics	10X Visium	FFPE		custom

Please click on the application or protocol of your choice for a more detailed information (ie sample requirements, experimental design considerations).
Illumina Sequencing Pricing
How to submit samples for Illumina/Short Read Sequencing.pdf
How to submit Ready-made Libraries.pdf