Table of contents
Instrument Reservation
Instrument reservations can only be made by employees. If you would like to make a reservation of an instrument, please check the availability of the corresponding instrument on the FGCZ Instrument Reservation System (IRS). If you find a slot which suits your plans, please contact your project coach or the person responsible for the corresponding instrument.Biophysics/Metabolomics technology platforms available at FGCZ
Metabolomics
MS Instruments
Name | Detailed informations by clicking * | Schema * | Mass analyzer | Dissociation | Max resolution @ m/z 200 & Max scan rate | Operation Modes* | Instruments at FGCZ - Service or User instrument |
QExactive | Hybrid quadrupole, Orbitrap | HCD | 140,000 & 12 Hz @ 17,500 resolution | DDA, Full MS, SIM, DIA, PRM | Service QEXACTIVE_3 and User QEXACTIVE_2 (shared with proteomics) | ||
QExactive HF | Hybrid quadrupole, Orbitrap | HCD | 240,000 & 18 Hz @ 15,000 resolution | DDA, Full MS, SIM, PRM, DIA, AIF, In-source fragmentation, Positive/negative ion switching, timed SIM | User/Service QEXACTIVEHF_2 | ||
TSQ Quantiva | Triple Quadrupole | HCD | Q1 and Q3 adjustable to 0.2 Da peak width (FWHM) across the entire mass range | Full MS, SIM, SRM, Reverse energy ramp MS/MS spectra (RER) | User/Service QUANTIVA_1 | ||
* • Full MS with high-resolution accurate mass detection • Selected Ion Monitoring (SIM) with high-resolution accurate-mass detection • Parallel Reaction Monitoring (PRM) for improved screening and quantitative confidence • Data-Independent Acquisition (DIA) for unbiased identification and quantitative analysis • In-source fragmentation of all ions • Positive/negative ion switching on chromatographic timescale • On-the-fly data-dependent decision making ddMS2 > data dependent acqusition (DDA) |
Other MS sources
Name | Detailed informations by clicking on the instrument * | Description | Operation Modes* | Instruments at FGCZ - Service/user lab access | |||
NanoMate Source | Nanoelectrospray ionization technology. Chip-based electrospray ionization technology from Advion. It combines the benefits of liquid chromatography, mass spectrometry, chip-based infusion, fraction collection, and direct surface analysis into one integrated ion source platform. | NanoMate |
Biophysics
Instruments
Name | Detailed informations by clicking * | Description | Instruments at FGCZ | ||||
PACBIO RS II | Single molecule sequencer used for SMFM (includes also the Rs2 150K upgrade | PacBio_RSII+ | |||||
Biacore T100 | Label-free protein interaction analysis | Biacore T100 | |||||
ProteOn™ XPR36 | The ProteOn™ XPR36 Protein Interaction Array System is an SPR optical biosensor that provides real-time data on the affinity, specificity, and interaction kinetics of protein interactions. | NA | |||||
Tecan HybStation | Automated Hybridisation Station (Computer controlled microreactor) | Tecan Hyb Station | |||||
Tecan LS400 Scanner | Laser Scanner (Confocal Microscope) | Tecan LS Scanner | |||||
Genetix QARRAY 2 | Microarray needle spotter Genetix QA2 | Tecan LS Scanner | |||||
Scienion SciFlexarrayer S11 | Piezoelectric microarray spotter Scienion S11 | Microarray spotter S11 | |||||
Please, contact Jens Sobek for further informations.
Others
Nanomate1-D Gel Electrophoresis
Flatbed Gel Scanner
Ultrospec Spectrophotometer
Qubit Fluorometer
pH Meter
DS-11 FX+ Spectrophotometer