Sequencing > Services

illumina MiSeq Personal Sequencer


FGCZ MiSeq Sequencing

Contact sequencing at fgcz.ethz.ch for planning your experiment.


Small Genome Sequencing
Exome sequencing
Targeted resequencing
ChIP seq
Amplicon Sequencing
miRNA profiling

Sequencing Throughput Specifications:

Read Length (bp)
Number and Type of Reads
1 x 36 (for smRNA seq) 15-17 M Single reads 540-610 Mb
2 x 25 20-25 M Paired-End reads 750-850 Mb
2 x 100 20-25 M Paired-End reads 3.0-3.4 Gb
2 x 150 20-25 M Paired-End reads 4.5-5.1 Gb
2 x 250 20-25 M Paired-End reads 7.5-8.5 Gb
2 x 300 20-25 M Paired-End reads 13-15 Gb

Library Preparation

One can use the following standard protocols:
  1. Illumina Library kits
  2. Nextera Kits
  3. Nextera XT for dual indexing

When do you opt for MiSeq

  • To acheive speed (Maximum run time is 40hrs)
  • When longer read lengths are required (2 x 300bp)
  • When the throughput required is lower than 10Gb
  • Want to sequence several plasmids in one go with 96 Barcodes (Using Nextera XT dual indexing)