Sequencing > Services

illumina MiSeq Personal Sequencer

FGCZ MiSeq Sequencing

Contact sequencing at fgcz.ethz.ch for planning your experiment.

Applications

Small Genome Sequencing
Exome sequencing
Targeted resequencing
ChIP seq
Amplicon Sequencing
miRNA profiling

Sequencing Throughput Specifications:

Read Length (bp)	Number and Type of Reads	Throughput
1 x 36 (for smRNA seq)	15-17 M Single reads	540-610 Mb
2 x 25	20-25 M Paired-End reads	750-850 Mb
2 x 100	20-25 M Paired-End reads	3.0-3.4 Gb
2 x 150	20-25 M Paired-End reads	4.5-5.1 Gb
2 x 250	20-25 M Paired-End reads	7.5-8.5 Gb
2 x 300	20-25 M Paired-End reads	13-15 Gb

Library Preparation

One can use the following standard protocols:

1. Illumina Library kits
2. Nextera Kits
3. Nextera XT for dual indexing

When do you opt for MiSeq

• To acheive speed (Maximum run time is 40hrs)
• When longer read lengths are required (2 x 300bp)
• When the throughput required is lower than 10Gb
• Want to sequence several plasmids in one go with 96 Barcodes (Using Nextera XT dual indexing)