Proteomics > Which service should I request? > Characterization of purified biomolecules
The determination of the mass of a protein/peptide is one way to confirm the identity of a protein and to provide insights into the presence of chemical / biological modifications. We offer analyses using (liquid chromatography) electrospray ionization (ESI) and matrix-assisted laser desorption/ionization (MALDI)-MS using routine or custom workflows.
If you don't find what you are looking for, or you are interested in special applications, please contact us at proteomics@fgcz.ethz.ch
Characterization of Purified Biomolecules
Table of contents
General description
After production and/or purification, proteins often need to be characterized for their integrity, the presence of natural or chemical modifications, or the assessment of sample heterogeneity. Antibody-drug conjugates, protein-metal complexes (i.e. metallothionein) and other biomolecules such as oligonucleotides can also be analyzed.The determination of the mass of a protein/peptide is one way to confirm the identity of a protein and to provide insights into the presence of chemical / biological modifications. We offer analyses using (liquid chromatography) electrospray ionization (ESI) and matrix-assisted laser desorption/ionization (MALDI)-MS using routine or custom workflows.
List of applications and services
FGCZ offers the following types of MS-based analyses for the Characterization of Purified BiomoleculesApplications (examples) | Which service to order |
Determination of the molecular weight of intact proteins | Protein / peptide mass determination |
Confirmation of protein / peptide sequence | Confirmation of protein identity |
Characterization of protein disulfide bridges | Disulfide bridge assignment |
Analysis of post-translational modifications (i.e. phospho, ubiquitin, acetyl, methyl) | Analysis of selected PTMs |
Characterization of protein-metal complexes (i.e. metallothioneins) | Protein-metal complexes |
Analysis of intact antibodies and antibody-drug conjugates | Antibody mass determination |
Identification of purified protein N-Terminal | N-terminal characterization (please contact us before ordering) |
Characterization of oligonucleotide sequences (<25000 Da) | Oligonucleotides mass determination |
Structural analysis of small molecules | Other (only in special cases - please contact us before ordering) |
Any analysis not covered in the list above | Other (please contact us before ordering) |
If you don't find what you are looking for, or you are interested in special applications, please contact us at proteomics@fgcz.ethz.ch
Quick links
- How to order an analysis?
- Follow our order instructions
- Questions?
- Check our FAQs page or contact us at proteomics@fgcz.ethz.ch
- Prices
- The prices for the most common proteomics services can be found here.