FGCZ NextSeq 500 Sequencing

Contact sequencing at fgcz.ethz.ch for planning your experiment.
Applications
Whole Genome sequencing
Exome sequencing
Targeted re-sequencing
Transcriptome Sequencing

Sequencing Throughput Specifications:
Read Length (bp) Number and Type of Reads Throughput
1 x 75 Up to 300 M Up to 20GB
2 x 75 Up to 600 M Paired-End reads Up to 45GB
2 x 150 Up to 600 M Paired-End reads Up to 90GB


Library Preparation
One can use the following standard protocols:
1. Illumina Library kits
2. Nextera Kits
3. Nextera XT for dual indexing

When do you opt for Nextseq 500
• To achieve speed (Maximum run time is 29 hrs)
• To sequence GC rich samples